Bio 1406 Enzyme Report

1) ABSTRACT The purpose of this sample was to carry forth the trend of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the prove was also to understand the relationship between the change in absorbance with time and the enzyme reply direct. To perform the experiment, the class was divided into groups. each group took on one of the factors affecting the enzyme. Before chaff the actual experiment, we apply the mixing tables that were given to us to pose up the test tubes with the appropriate substances. We followed the same procedure for individually experiment variable (1-5). We then rid the cell of hepatotoxic hydrogen peroxide (H2O2) so the cell would not determine damaged, as stated in our lab manual. For every empiric variable, test tube 1 was always the control. Once the solutions were post we mixed them and poured them into the cuvette and utilize the spectrophotometer machine to run t he enzyme and determine its wander and its absorbance. When the actual experiment was done we all shared the results and graphed them. 2) doorway Before beginning the actual experiment we had to become well-known(prenominal) with a spectrometer machine and its functions.

It is basically an instrument used to measure the amount of light absorbed by a solution (Frankhauser, David B). As stated in our lab manual, the darker the solution, the great its absorbance. The reason why we used this machine was to figure let on the relationship between the change in absorbance with time and the enzyme reaction ra te. The enzyme reaction rate was determined ! when spots of absorbance versus time were constructed. The slope of all(prenominal) plot was related to the reaction rate. After running a keen experiment to learn how to use the spectrophotometer, we learned or so the background information of enzymes. Enzymes are proteins that serve as atom smasher (Ophardt, Charles E.). By definition, a catalyst is a substance that initiates or accelerates...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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